![]() If this option is enabled, normalization and Note that normalization of RGB images is not supported. The maximum range is 0-255 for 8-bit images and 0-65535 for 16-bit images. The range is equal to the maximum range for the data type, or 0-1.0 for float images. Zero to prevent a few outlying pixel from causing the histogram stretch to not work as intended.Ĭheck Normalize and ImageJ will recalculate the pixel values of the image so Increasing this value increases contrast. Saturated pixels determines the number of pixels in the image that are allowed This command does not alter pixel values as long as the Normalize andĮqualize histogram options are not enabled. Macro demonstrates how to add particles found by Find Maxima to the ROI Manager.Įnhances image contrast by using either histogram stretching or histogram equalization.īoth methods are described in detail in the Macro runs it on all the images in a stack and creates a second stack containing the output images. "Exclude Edge Maxima" applies to the maximum, not to the particle.įind Maxima does not work on stacks, but the Particles touching the edge if "Exclude Edge Maxima" is selected. Note that "Segmented Particles" will usually result in In the output image does not depend on the "Output Type" selected. The number of particles (as obtained by "Analyze Particles") The "Black Background" option in Process>Binary>Options. Output is a binary image, with foreground 255 and background 0, using an inverted or normal LUT depending on "Output Type", the area below the lower threshold is considered a background. The upper threshold of the image is ignored.
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